PKI-587

PKI-587 and Sorafenib Targeting PI3K/AKT/mTOR and Ras/Raf/MAPK Pathways Synergistically Inhibit HCC Cell Proliferation
Roberto Gedaly, M.D.,*,1 Paul Angulo, M.D.,† Jonathan Hundley, M.D.,* Michael F. Daily, M.D.,* Changguo Chen, Ph.D.,* and B. Mark Evers, M.D.*,‡
*Department of Surgery; †Department of Internal Medicine; and ‡Markey Cancer Center, University of Kentucky, College of Medicine, Lexington, Kentucky

Originally submitted August 18, 2011; accepted for publication October 27, 2011

Background. Deregulated Ras/Raf/MAPK and PI3K/ AKT/mTOR signaling pathways are found in hepato- cellular carcinoma (HCC). This study aimed to test the inhibitory effects of PKI-587 and sorafenib as single agents or in combination on HCC (Huh7 cell line) proliferation.
Materials and Methods. 3H-thymidine incorpora- tion and MTT assay were used to assess Huh7 cell proliferation. Phosphorylation of the key enzymes in the Ras/Raf/MAPK and PI3K/AKT/mTOR pathways was detected by Western blot.
Results. We found that PKI-587 is a more potent PI3K/mTOR inhibitor than PI-103. Combination of PKI-587 and sorafenib was a more effective inhibitor of Huh7 proliferation than the combination of PI-103 and sorafenib. Combination of PKI-587 and sorafenib synergistically inhibited epidermal growth factor (EGF)-stimulated Huh7 proliferation compared with monodrug therapy. EGF increased phosphorylation of Ras/Raf downstream signaling proteins MEK and ERK; EGF-stimulated activation was inhibited by sora- fenib. However, sorafenib, as a single agent, increased AKT (Ser473) phosphorylation. EGF-stimulated AKT (ser473) activation was inhibited by PKI-587. PKI-587 is a potent inhibitor of AKT (Ser473), mTOR (Ser2448), and S6K (Thr389) phosphorylation; in contrast, rapamycin stimulated mTOR complex 2 substrate AKT(Ser473) phosphorylation although it inhibited mTOR complex 1 substrate S6K phosphoryla- tion. PKI-587, as a single agent, stimulated MEK and ERK phosphorylation. However, when PKI-587 and sorafenib were used in combination, they inhibited

1 To whom correspondence and reprint requests should be addressed at Department of Surgery, University of Kentucky, College of Medicine, 800 Rose Street, Room C453, Lexington, KY 40536-0293. E-mail: [email protected]
all the tested kinases in the Ras/Raf /MAPK and PI3K/ AKT/mTOR pathways.
Conclusion. The combination of PKI-587 and sorafe- nib has the advantage over monodrug therapy on inhibition of HCC cell proliferation by blocking both PI3K/AKT/mTOR and Ras/Raf/MAPK signaling pathways. © 2012 Elsevier Inc. All rights reserved.
Key Words: epidermal growth factor; sorafenib; PKI-587; PI-103; rapamycin; mTOR complex 1; mTOR complex 2; negative feedback loop.

INTRODUCTION

Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver; it ranks fifth among the most diagnosed cancers and represents the third most common cause of cancer-related deaths world- wide [1].
Recent publications indicate that HCC cell activation by different factors is known to involve several signal- ing pathways, including the Ras/Raf/MAPK pathway, the PI3K/AKT/mTOR pathway, the WNT/b-Catenin pathway, the Hedgehog pathway, and the Hippo tumor suppression pathway [2, 3]. Among them, the Ras/Raf/ MAPK and the PI3K/AKT/mTOR pathways are the most critical pathways in the development and prolifer- ation of HCC and have been extensively investigated. The Ras/Raf/MAPK pathway is typically activated in HCC as a result of increased signaling induced from up- stream growth factors and due to inactivation of tumor suppressor genes [4]. The PI3K/AKT/mTOR signaling pathway plays an important role in HCC and is acti- vated in 30%–50% of HCC. The ribosomal protein S6 (RPS6), a target of p70S6K, is aberrantly activated in 50% of HCC [5]. Despite enormous efforts, the etiology

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of HCC tumorigenesis, progression, and recurrence remains unclear. There is a critical need to identify more effective regimens to treat HCC and to prevent tumor recurrence.
The anti-cancer drug sorafenib is a multi-kinase inhibitor of more than a dozen kinases at high potency [6]. Sorafenib has been shown to inhibit tumor cell pro- liferation by inhibiting the Ras/Raf/MAPK pathway and to suppress angiogenesis by blocking VEGFR and PDGFR signaling. A recent clinical trial evaluating the treatment of advanced HCC using sorafenib has ob- tained promising results [7]. However, sorafenib does not directly block the PI3K/AKT/mTOR pathway in HCC. It was reported that sorafenib could even increase phosphorylation of mTOR targets (S6K and 4EBP1) [8] and to activate mTOR complex 2 activity [9]. To over- come this problem, sirolimus (a rapalog) has been used by other researchers in combination with sorafe- nib to target the PI3K/AKT/mTOR pathway. Our group recently used PI-103, a dual PI3K/mTOR inhibitor, in combination with sorafenib to inhibit HCC prolifera- tion and found that the two drugs can inhibit HCC synergistically by blocking both Ras/Raf/MAPK and the PI3K/AKT/mTOR pathways [9]. Recently, a novel drug PKI-587, which has similar mechanism of action as PI-103, has demonstrated potent inhibitory effects on several human cancer cell lines such as melanoma, glioma, lung, colon, and breast cancers [10] in preclini- cal studies. PKI-587 has been approved by the FDA and is currently being evaluated in a phase 1 clinical trial. PKI-587 is a chemically synthesized small molecule that specifically inhibits PI3K class-IA and mTOR complex 1 (mTORC1) and complex 2 (mTORC2) [11]. The aim of this study was to evaluate the antiprolifera- tive effect of PKI-587 and sorafenib, as single agents and in combination, and their effects on Ras/Raf/ MAPK and PI3K/AKT/mTOR signaling pathways.

MATERIALS AND METHODS

Cell Culture

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The human HCC cell line, Huh7, was cultured in DMEM (Cat# 12100-046) medium (Invitrogen, Carlsbad, CA) 10% heat inactivated FBS in a 37◦C incubator with 5% CO2 in the air.

Chemicals and Antibodies

PKI-587 was a generous gift from Pfizer Inc. (New York, NY). Other chemicals and antibodies were purchased as described previously [9].

3H-Thymidine Incorporation Assay
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Huh7 cells were plated in 96-well plates at 1000 cells/well in 0.2 mL DMEM 10% FBS and treated with various concentrations of the sorafenib and PKI-587, as single agents or in combination, and cultured for 72 h. 3H-thymidine incorporation assay was performed according to our published methods [9].
MTT Assay
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Huh7 cells were plated in 96-well plates at 5000 cells/well (n 12) in 100 mL of DMEM 10%FBS and cultured for 24 h. The cells were then cultured in 1% FBS or in 10% FBS in DMEM for 24 h. EGF, sorafenib, or PKI-587 was then added to the cells and cultured for an- other 48 h. Carrier DMSO was used as a vehicle control (<0.1% final concentration). MTT assay was performed as previously described [9].

Western Blot

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Huh7 cells were cultured in DMEM 10%FBS in 100 3 20 mm tissue culture dishes until w70% confluent. The cells were treated with sorafenib (10 mM), PKI-587(1 mM), rapamycin (20 nM), for 1h, then treated with EGF (6.5 nM) for 15 min. All the other procedures were performed as previously described [9].

Statistical Analysis

All analyses were performed using the software SPSS ver. 18 (SPSS Inc., Chicago IL). Data are presented as mean 6 SE. For nominal data, ANOVA followed by Tukey multiple range test was used; for two groups of continuous data, paired t-test was used. The level of statistical significance was set at P < 0.05.

RESULTS

PKI-587 More Potently Inhibits Huh7 Proliferation than PI-103

Huh7 cells were treated with PKI-587 or PI-103 at various concentrations (0–1000 nM). The results of 3H-thymidine incorporation indicated the IC50 (50% Inhibition concentration) of PKI-587 was 39 nM; the IC50 of PI-103 was 368 nM. Therefore, PKI-587 is about nine times more potent than PI-103 (Fig. 1).
We also found that the combination of PKI-587 with sorafenib was more potent than the combination of PI-103 and sorafenib on inhibition of Huh7 prolifera- tion (Fig. 2).
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EGF-stimulated Huh7 proliferation in DMEM 1% FBS or in DMEM 10% FBS was synergistically and significantly inhibited by PKI-587 and sorafenib.
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Since 3H-thymidine incorporation detects DNA synthesis in a short time period, we used MTT assay to determine if sorafenib and PKI-587 can inhibit Huh7 proliferation over a longer period of treatment. We found that EGF, at a concentration of 2.5 nM, optimally stimulates Huh7 proliferation in both culture conditions (DMEM 1% or 10% FBS). When the cells were cultured in 1% FBS, EGF (2.5 nM) stimulated Huh7 proliferation 42% compared with control (P < 0.01; n 12). The EGF-stimulated Huh7 prolifer-
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ation was inhibited 69% (P < 0.001; n 12) by sorafenib
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(at 2.5 mM). PKI-587 (0.5 mM) inhibited EGF-stimulated Huh7 proliferation 57% (P < 0.001; n 12). Combina- tion of the PKI-587 and sorafenib synergistically inhibited EGF-stimulated Huh7 proliferation by 81% (P < 0.001; n 12). The effect of the drug combination was significantly different from the inhibitory effect of

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% of 3H-Thymidine Uptake
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FIG. 1.

200 400 600 800 1000
Concentrations (nM)
Comparison of PKI-587 and PI-103 on inhibition of Huh7
tion 48% (P < 0.001, n 12). The combination of PKI-587 and sorafenib synergistically inhibited EGF-stimulated Huh7 proliferation 55% (P < 0.001; n 12). The effect of combination of the two drugs was significantly different from the inhibitory effect of sorafenib (P < 0.01, n 12) or PKI-587 (P 0.03, n 12) (Fig. 3 A, B). The experiments were repeated with similar results.
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The combination of PKI-587 and sorafenib inhibited multiple key enzymes in the Ras/Raf/MAPK and PI3K/AKT/mTOR signaling pathways.
In comparison with DMSO vehicle control, EGF stim- ulated mTOR(Ser2448), S6K(Thr389), AKT(Ser473),
MEK1/2(Ser217/221), and ERK1/2(Thr202/204) phos- phorylation 21%, 25%, 93%, 28%, and 23%, respec-

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¼ þ
3H-thymidine incorporation. The Huh7 cells were plated to 96-well
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plates at 1000 cells/well in 0.2 mL DMEM 10% FBS with five repeats (n 5). The cells were treated with various concentrations of the drugs (as indicated) and cultured for 72 h. Carrier DMSO (<0.1% final concentration) was added to the zero controls. The cells were pulsed with methyl-3H-thymidine (specificity 2 Ci/mmole) for 4 h at 1 mCi/ well. 3H-thymidine incorporation was measured by scintillation
counting in a Packard Scintillation Analyzer (model TRI-CARB 2100TR). (*PKI-587 caused inhibition is significantly more severe than PI-103 caused inhibition at the indicated concentrations, P < 0.02). IC50 (50% inhibition concentration) of PKI-587 was 39
nM; IC50 of PI-103 was 368 nM. PKI-587 at all concentrations tested
(15.6–1000 nM) significantly inhibited Huh7 proliferation compared with control (P < 0.001). PI-103 from concentrations of 62.5–1000 nM significantly inhibited Huh7 proliferation compared with control (P < 0.008).

¼
¼
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sorafenib (P 0.036) or PKI-587 (P < 0.01). When the cells were cultured in 10% FBS, EGF (2.5 nM) stimu- lated Huh7 proliferation 20% (P < 0.01; n 12). The EGF-stimulated Huh7 proliferation was inhibited 23% by sorafenib (at 2.5 mM) (P < 0.01; n 12). PKI- 587 (0.5 mM) inhibited EGF-stimulated Huh7 prolifera-

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3H-Thymidine Uptake (%)
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80

60

40

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Sorafenib (µM) 0 0.63 1.25 2.5 5 10
PKI-587 (nM) 0 15.6 31.2 62.5 125 250
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PI-103 (nM) 0 15.6 31.2 62.5 125 250
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FIG. 2. Combination of PKI-587 and sorafenib caused more potent inhibition of Huh7 3H-thymidine incorporation than the com- bination of PI-103 and sorafenib did. Cell culture and 3H-thymidine incorporation were done as indicated in Figure 1. *PKI-587 sorafe-
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nib caused more inhibition than PI-103 sorafenib (P < 0.037). Drug combinations at all drug concentrations inhibited Huh7 3H-thymidine incorporation significantly compared with vehicle control (P < 0.05).
tively. Sorafenib at 10 mM inhibited EGF-stimulated

FIG. 3. PKI-587 and sorafenib inhibited EGF stimulated Huh7 proliferation detected by MTT assay. (A) Cells cultured in 1% FBS starvation condition. Huh7 cells were plated in 96-well plate at 5000 cells/well (n 12) in 100 mL of DMEM 10% FBS and cultured for 24 h. The cells were starved for 24 h in DMEM 1% FBS. Then the cells were cultured in 1% FBS in DMEM for 48 h with DMSO (0.017%, control), EGF, and inhibitors at the indicated concentra- tions. (B) Cells cultured in 10% FBS. Huh7 cells were plated in 96-well plate at 5000 cells/well (n 12) in 100 mL of DMEM 10% FBS and cultured for 24 h. EGF and inhibitors were added and cells cultured for another 48 h.

MEK1/2 and ERK1/2 phosphorylation 79% and 81%, re- spectively. However, sorafenib, as a single agent, only slightly inhibited S6K phosphorylation (12%) but did not inhibit EGF-stimulated mTOR and AKT phosphor- ylation in the PI3K/AKT/mTOR signaling pathway. In- terestingly, we found that sorafenib dramatically increased AKT phosphorylation 147% compared with EGF treatment. PKI-587 at 0.5 mM, as a single agent, in- hibited EGF stimulated mTOR(Ser2448), S6K(Thr389) (marker for mTORC1 activity), and AKT(Ser473) (marker for mTORC2 activity) phosphorylation 35%, 98.2%, and 98%, respectively, thus suggesting PKI-587 inhibits both mTORC1 and mTORC2 activity. Rapamycin, as a single agent, inhibited mTOR(Ser2448), and S6K(Thr389) phosphorylation 3% and 97%, but rapamycin did not inhibit EGF-stimulated AKT(Ser473) phosphorylation. In- stead, rapamycin increased mTORC2 substrate AKT(Ser473) phosphorylation by 57%. Even more sig- nificantly, the combination of sorafenib and rapamycin increased AKT phosphorylation 120% compared with EGF treatment. Rapamycin also increased MEK1/ 2(Ser217/221) and ERK1/2(Thr202/204) phosphoryla- tion 31% and 54%. PKI-587, as a single agent, did not inhibit EGF-stimulated Ras/Raf/MAPK pathway and was found to increase MEK1/2(Ser217/221) and ERK1/2(Thr202/204) phosphorylation by 30% and 52%. The combination of PKI-587 and sorafenib strongly inhibited both Ras/Raf/MAPK and PI3K/ AKT/mTOR signaling pathways. The EGF-stimulated mTOR(Ser2448), S6K(Thr389), AKT(Ser473), MEK1/
2(Ser217/221), and ERK1/2(Thr202/204) phosphoryla- tion was inhibited 80%, 93%, 98%, 45%, and 48%, respectively (Fig. 4A and B). These findings indicate that combination treatment with PKI-587 and sorafenib has a significant advantage over single agent therapy at specific concentrations by preventing unde- sirable increased activity of non-targeted pathways.

DISCUSSION

Recently, our group reported the importance of dual inhibition of PI3K and mTOR, key enzymes of the PI3K/AKT/mTOR signaling pathway, using PI-103 in combination with sorafenib to treat HCC. We found a synergistic effect with PI-103 and sorafenib combina- tion treatment in the Huh7 HCC cell line. Interestingly, we demonstrated that blockage/inhibition of only one of the main pathways PI3K/mTOR or Ras/Raf/MAPK, separately, can result in activation of the other path- way. This could explain why combined inhibition of these two pathways produces a more significant inhibi- tion of HCC cell proliferation. Based on these findings, we wished to study another dual inhibitor of the PI3K/
mTOR pathway in vitro and then develop an in vivo xenograft model. Initially, we tested potency comparing PKI-578 alone and in combination with sorafenib. Our experiments found that PKI-587, as single agent, is more potent than PI-103 in the inhibition of HCC prolif- eration; the combination of PKI-587 and sorafenib is more efficient than the combination of PI-103 and sora- fenib. The main reason PKI-587 is more efficacious than PI-103 may be related to biostability of the molecule. It has been reported that PI-103 is not a very stable molecule and it suffers from extensive metabolism [12]. Due to the potency, bioavailability, and biosafety profile of PKI-587 and its recent approval by FDA in a phase 1 clinical trial, we decided to study this drug and its inhibitory effects on HCC cells.
Our 3H-thymidine incorporation and MTT assay experiments indicated that PKI-587 or sorafenib, as single agents, inhibits HCC cell proliferation. Combina- tion of PKI-587 and sorafenib resulted in synergistic inhibition of HCC cell proliferation when cells were cul- tured in 1% or 10% FBS. The use of 1% FBS allows the cells to reach a quiescent state after 24 h of starvation, so that EGF stimulation is more prominent. In addi- tion, this condition mimics the limited nutrition from poor blood supply in tumor cells. The synergistic inhibi- tion by the drug combination treatment indicates that the Ras/Raf/MAPK and PI3K/AKT/mTOR pathways play an important role in HCC cell proliferation. This was confirmed by Western blot experiments showing inhibition of key enzymes in these two pathways. We found that EGF stimulates MEK and ERK phosphory- lation in the Ras/Raf/MAPK pathway and enhances mTOR, AKT, and S6K phosphorylation in the PI3K/ AKT/mTOR pathway, in agreement with previously published studies [2, 13]. PKI-587 monotherapy strongly inhibited EGF stimulated mTOR, S6K, and AKT (Ser473) phosphorylation. In contrast, rapamycin only inhibited mTOR (3%) and S6K (93%) phosphoryla- tion, but increased AKT (Ser473) phosphorylation 57%. S6K phosphorylation is a marker of mTOR complex 1 (mTORC1) activity, while AKT(Ser473) phosphoryla- tion is a marker of mTOR complex 2 (mTORC2) activity [14]. We demonstrated a different inhibition pattern be- tween PKI-587 and rapamycin. PKI-587 inhibited both mTORC1 and mTORC2; however, rapamycin only in- hibited mTORC1. As noted in our previous experiments with PI-103, PKI-587, another dual PI3K/mTOR inhib- itor, as single agent, stimulated MEK and ERK phos- phorylation. This may be explained by the fact that inhibition of mTORC1 can eliminate the S6K-mediated negative feedback inhibitory loop, caus- ing activation of Ras/Raf/MAPK pathway [9, 15]. Sorafenib, as single agent, inhibited EGF-stimulated MEK and ERK phosphorylation, but interestingly it slightly stimulated mTOR and dramatically stimulated

FIG. 4. PKI-587 and sorafenib differentially inhibited or activated phosphorylation of several key enzymes in the Ras/Raf/MAPK and PI3K/ Akt/mTOR pathways. (A) Western blot for p-mTOR (Ser2448), p-S6K (Thr389), p-AKT (Ser473), p-MEK1/2(Ser217/221), and p-ERK1/ 2(Thr202/204) level after different treatments. Also shown are endogenous mTOR, S6K, AKT, MEK1/2, and ERK1/2. Positive and negative controls for p-MEK and for p-ERK were ordered from Cell Signaling Technology (Danvers, MA) and included in the experiments but are not shown. All the proteins except ERK1/2 were detected from the same nitrocellulose membrane. Phosphorylated kinase band densities were assessed by Scion Image software and normalized by b-actin. (B) A plot generated based on b-actin normalized phosphorylated kinase band densities in (A). The b-actin normalized density of each phosphorylated kinases in EGF treatment is calculated and set at 100 arbitrary units and the density of phosphorylated kinases in the other treatments are as percentage of the EGF treatment for easy comparison. For simplicity, the ‘‘nothing added’’ treatment is not included in (B).

AKT (Ser473) phosphorylation, and only had minor effect on EGF stimulated S6K phosphorylation. These results confirm our previous findings that inhibition of one of the pathways may cause stimulation of the other main pathway in HCC cells. This supports the hy- pothesis that monotherapy in HCC treatment may not be appropriate. Presumably, sorafenib may induce other signals, such as HGF/HGFR, that further stimu- lates the EGF-stimulated PI3K/AKT/mTOR pathway. Indeed, sorafenib stimulated HGF secretion in HCC cells and promoted c-Met, S6K, and 4EBP1 phosphory- lation [8]. Increased HCC activation and mobility by HGF were reported to be mediated through PI3K sig- naling [16]. Sorafenib and rapamycin as monotherapy, both enhanced EGF stimulated AKT (Ser473) phos- phorylation; PKI-587 caused near complete inhibition

of EGF-stimulated AKT (Ser473) phosphorylation. Since AKT plays an important role in tumor cell sur- vival and is involved in Hippo (Mst1/Mst2) signaling [17, 18], AKT may be a target in HCC therapy.
Recent studies have found that the Ras/Raf/MAPK and PI3K/AKT/mTOR are the main activated pathways in HCC cells [2]. Several attempts have been made to target these pathways as therapy. Villanueva et al. used a rapamycin analogue, everolimus, and an EGF/ VEGF inhibitor, AEE788, to block these two pathways in the Huh7 HCC cell line, which resulted in the inhibi- tion of mTOR signaling in vitro and decreased tumor progression and increased survival in a xenograft model. The combination of everolimus and AEE788 en- hanced tumor suppression compared with everolimus monotherapy [19]. Newell et al. used sorafenib and

rapamycin to target mTOR and Ras/Raf/MAPK signal- ing and decreased proliferation and induced apoptosis in HCC cell lines. In a xenograft mouse model, the com- bination of rapamycin and sorafenib enhanced tumor necrosis and ulceration compared with sorafenib alone [13]. The failure of a novel MEK inhibitor (AZD6624) to show any clinical benefits in HCC treatment indicates that inhibition of Ras/Raf/MAPK signaling pathway alone is not sufficient in HCC therapy [20]. To date, the use of sorafenib in advanced HCC has become common practice. Llovet et al. [7] demonstrated that sorafenib has a significant suppression on tumor pro- gression and patient survival in patients with advanced HCC. Our current study analyzing PKI-587 and sorafe- nib on inhibition of HCC proliferation generated simi- lar results compared with our previous study with PI-103 and sorafenib [9]; however, PKI-587 was found to have higher potency than PI-103.
The use of mTOR inhibitors such as rapamycin to treat HCC is still in debate and faces many challenges. It was reported that prolonged treatment with rapamy- cin could inhibit both mTORC1 and mTORC2 in several cancer cell lines; however, no inhibition of mTORC2 in the HCC cell line was reported [21]. Rapamycin dis- rupts the assembly of mTORC1 but has no effect on mTORC2 conformation. Because of this, more free- mTOR units are available to bind rictor and mSin1 to generate more mTORC2. mTORC2 phosphorylates AKT at Ser473 and promotes tumor cell survival and in- duces resistance to contemporary mTOR inhibitors [22, 23]. Inhibition of mTORC1 by rapamycin can eliminate the S6K-mediated negative feedback inhibitory loop with subsequent activation of upstream PI3K and Ras/Raf/MAPK pathways [15, 24]. An agent that could inhibit both mTORC1 and mTORC2, and also block up- stream of mTOR, will result in a stronger inhibition of the PI3K/AKT/mTOR pathway. The novel drug PKI-587 exhibits potent dual inhibition of PI3K and mTOR (mTORC1 and mTORC2). It has high bioavail- ability and biosafety. PKI-587 has been used in preclin- ical studies to inhibit 50 human cancer types [10, 11]. PKI-587 in combination with MEK1/2 inhibitor (PD0325901) produced more tumor suppression effect than PKI-587 monodrug therapy [10]. This finding strongly supports the idea to use PKI-587 and sorafenib in combination. So far, PKI-587 has not been used in HCC preclinical or clinical studies. We realize that although we are the first group to use PKI-587 in com- bination with sorafenib in HCC study, the functional mechanism of PI-103 and PKI-587 on inhibition of HCC is similar. However, PKI-587 has at least two sig- nificant advantages that we have identified. In addition to being more potent, PKI-587 is FDA approved, cur- rently under investigation on a phase 1 clinical trial in humans. Working with an FDA approved drug would
allow us in a shorter period of time to translate our preclinical research into a phase 1 clinical trial to treat advanced HCC in humans. We have not yet tested these drugs in other HCC cell lines. We are planning to use Hep3B and PLC HCC cell lines to compare drug inhibi- tion patterns with Huh7. Hep3B and PLC both express HBV surface antigen, and Huh7 expresses delta anti- gen. The Hep3B cell line is p53 negative, PLC is p53 reduced, and Huh7 has increased p53 protein level [25, 26]. We are also trying to find a HCC cell line with Ras/Raf mutation that could demonstrate different patterns of response to PKI-587 and sorafenib.
Our future investigation will be focused on in vivo studies to translate our in vitro results. Based on our in vitro experiments and on other researchers’ pub- lished data, the dosage of PKI-587 in HCC xenograft mouse model would be 5–50 mg/kg every 4 d. This dos- age was reported by Mallon et al. in breast cancer, colon cancer, and glioma xenograft mouse model using PKI-587 [10]. At this dosage no toxicity was reported. Sorafenib was used at 10-100 mg/kg every day in xenograft mouse model with no toxicity reported [27].
In conclusion, PKI-587 and sorafenib are potent anti-HCC drugs; their application in combination has significant advantages compared with monodrug treatment in the inhibition of HCC pivotal pathways Ras/Raf/MAPK and PI3K/AKT/mTOR.

REFERENCES
⦁ Llovet JM, Burroughs A, Bruix J. Hepatocellular carcinoma. Lancet 2003;362:1907.
⦁ Llovet JM, Bruix J. Molecular targeted therapies in hepatocellu- lar carcinoma. Hepatology 2008;48:1312.
⦁ Lachenmayer A, Hoshida Y, Llovet JM. Hippo tumor suppressor pathway: Novel implications for the treatment of hepatocellular carcinoma. Gastroenterology 2010;139:692.
⦁ Strumberg D. Preclinical and clinical development of the oral multikinase inhibitor sorafenib in cancer treatment. Drugs Today (Barc) 2005;41:773.
⦁ Minguez B, Tovar V, Chiang D, et al. Pathogenesis of hepatocel- lular carcinoma and molecular therapies. Curr Opin Gastroen- terol 2009;25:186.
⦁ Chaparro M, Gonzalez ML, Trapero-Marugan M, et al. Review article: Pharmacological therapy for hepatocellular carcinoma with sorafenib and other oral agents. Aliment Pharmacol Ther 2008;28:1269.
⦁ Llovet JM, Ricci S, Mazzaferro V, et al. Sorafenib in advanced hepatocellular carcinoma. N Engl J Med 2008;359:378.
⦁ Huynh H, Ngo VC, Koong HN, et al. Sorafenib and rapamycin induce growth suppression in mouse models of hepatocellular carcinoma. J Cell Mol Med 2009;13:2673.
⦁ Gedaly R, Angulo P, Hundley J, et al. PI-103 and sorafenib inhibit hepatocellular carcinoma cell proliferation by blocking Ras/Raf/MAPK and PI3K/AKT/mTOR pathways. Anticancer Res 2010;30:4951.
⦁ Mallon R, Feldberg LR, Lucas J, et al. Antitumor efficacy of PKI-587, a highly potent dual PI3K/mTOR kinase inhibitor. Clin Cancer Res 2011;17:3193.
⦁ Venkatesan AM, Dehnhardt CM, Delos SE, et al. Bis(morpho- lino-1,3,5-triazine) derivatives: Potent adenosine 5’-triphos- phate competitive phosphatidylinositol-3-kinase/mammalian

target of rapamycin inhibitors: Discovery of compound 26 (PKI- 587), a highly efficacious dual inhibitor. J Med Chem 2010; 53:2636.
⦁ Raynaud FI, Eccles SA, Patel S, et al. Biological properties of potent inhibitors of class I phosphatidylinositide 3-kinases: From PI-103 through PI-540, PI-620 to the oral agent GDC- 0941. Mol Cancer Ther 2009;8:1725.
⦁ Newell P, Toffanin S, Villanueva A, et al. Ras pathway activation in hepatocellular carcinoma and anti-tumoral effect of combined sorafenib and rapamycin in vivo. J Hepatol 2009;51:725.
⦁ Yu K, Shi C, Toral-Barza L, et al. Beyond rapalog therapy:
Preclinical pharmacology and antitumor activity of WYE- 125132, an ATP-competitive and specific inhibitor of mTORC1 and mTORC2. Cancer Res 2010;70:621.
⦁ Carracedo A, Ma L, Teruya-Feldstein J, et al. Inhibition of mTORC1 leads to MAPK pathway activation through a PI3K- dependent feedback loop in human cancer. J Clin Invest 2008; 118:3065.
⦁ Nakanishi K, Fujimoto J, Ueki T, et al. Hepatocyte growth fac- tor promotes migration of human hepatocellular carcinoma via phosphatidylinositol 3-kinase. Clin Exp Metastasis 1999; 17:507.
⦁ Kim D, Shu S, Coppola MD, et al. Regulation of proapoptotic mammalian ste20-like kinase MST2 by the IGF1-Akt pathway. PLoS One 2010;5:e9616.
⦁ Yuan Z, Kim D, Shu S, et al. Phosphoinositide 3-kinase/Akt inhibits MST1-mediated pro-apoptotic signaling through phosphorylation of threonine 120. J Biol Chem 2010; 285:3815.
⦁ Villanueva A, Chiang DY, Newell P, et al. Pivotal role of mTOR signaling in hepatocellular carcinoma. Gastroenterology 2008; 135:1972.
⦁ Faivre S, Bouattour M, Raymond E. Novel molecular therapies in hepatocellular carcinoma. Liver Int 2011;31(Suppl 1):151.
⦁ Sarbassov DD, Ali SM, Sengupta S, et al. Prolonged rapamycin treatment inhibits mTORC2 assembly and Akt/PKB. Mol. Cell 2006;22:159.
⦁ Sarbassov DD, Guertin DA, Ali SM, et al. Phosphorylation and regulation of Akt/PKB by the rictor–mTOR complex. Science 2005;307:1098.
⦁ Zou CY, Smith KD, Zhu QS, et al. Dual targeting of AKT and mammalian target of rapamycin: A potential therapeutic approach for malignant peripheral nerve sheath tumor. Mol Cancer Ther 2009;8:1157.
⦁ Wan X, Harkavy B, Shen N, et al. Rapamycin induces feedback activation of Akt signaling through an IGF-1R-dependent mechanism. Oncogene 2007;26:1932.
⦁ Chang J, Hsu Y, Kuo P, et al. Increase of Bax/ Bcl-XL ratio and arrest of cell cycle by luteolin in immortalized human hepatoma cell line. Life Sci 2005;76:1883.
⦁ Su SJ, Chow NH, Kung ML, et al. Effects of soy isoflavones on ap- optosis induction and G2-M arrest in human hepatoma cells involvement of caspase-3 activation, Bcl-2 and Bcl-XL down- regulation, and Cdc2 kinase activity. Nutr Cancer 2003;45:113.
⦁ Liu L, Cao Y, Chen C, et al. Sorafenib blocks the RAF/MEK/ERK pathway, inhibits tumor angiogenesis, and induces tumor cell apoptosis in hepatocellular carcinoma model PLC/PRF/5. Cancer Res 2006;66:11851.

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