This investigation sought to determine the prevalence and antimicrobial resistance patterns of Salmonella species. Poultry meat, intended for human consumption, was kept separate. Between 2019 and 2021, 145 samples were analyzed under the regulations established in ISO 6579-12017. The strains isolated were characterized by biochemical-enzymatic assays and serotyping, specifically applying the Kauffmann-White-Le Minor scheme. The susceptibility of the antibiotics was determined through the use of the Kirby-Bauer method. Forty Salmonella bacteria were isolated. Isolated strains were subjected to serotyping, which identified Salmonella Infantis as the dominant species. S961 cost A considerable 80% of the isolated bacterial strains exhibited multidrug resistance, specifically *S. Infantis*. Poultry meat samples have shown the presence of circulating MDR Salmonella, with the study pinpointing the prevalence of S. Infantis serovar, thereby indicating a developing health risk under the One Health model.
A 13-month study evaluated the use of an electrochemical (impedance) tool to track Escherichia coli levels in shellfish. This study's central purpose was to compare the standard most probable number (MPN) and -trac 4200 (log imped/100 g) measurements for E. coli contamination (log MPN/100 g) in non-depurated bivalve mollusks (BM) from five sampling areas along the Veneto-Emilian coast in Italy, encompassing a total of 118 samples. The secondary objective was to ascertain the relationship between E. coli concentrations in BM and environmental factors, utilizing a substantial dataset of 690 observations. The methods demonstrated a moderate, positive correlation, as evidenced by Pearson and Spearman coefficients of 0.60 and 0.69, respectively, at a statistically significant level (P<0.0001, MPN/100g: 4600). The findings indicated the impedance method's efficacy for speedy evaluation and regular utilization, notably in clams, but demonstrated a lesser impact on Mytilus. E. coli load prediction was enabled by models developed using multivariate permutational variance analysis and multinomial logistic regression, which identified suitable environmental factors. E. coli contamination exhibited a correlation with salinity and seasonal changes; however, hydrometry and local salinity had a more pronounced effect on the localized prevalence. The impedance method, in conjunction with environmental data analysis, can assist purification phase management in complying with legal restrictions. This enables local control authorities to develop preventive measures in reaction to the effects of climate change, specifically concerning extreme meteorological events.
The widespread availability of microplastics (Ps) to aquatic organisms, from zooplankton up to top predators, poses a significant new challenge to the marine environment. genetic program This study sought to assess a technique for extracting microplastics from the gastrointestinal systems of 122 Sepia officinalis, captured in the Adriatic Sea (along Abruzzo's coastal areas), to determine their abundance in this species, which has received limited prior investigation. To extract gastrointestinal content, a 10% potassium hydroxide solution was the method employed. A study on wild animal populations revealed the presence of microplastics in 98 of 122 specimens (80.32%, confidence interval 7327-8737%), with an average of 682,552 particles per subject. Black fragments, as noted by numerous authors, were the most prevalent among the collected pieces; however, isolated instances of blue fibers and transparent orbs were also observed. This study, aligning with earlier investigations, emphasizes the crucial necessity of addressing the extensive dispersion of microplastics throughout the marine environment, including surfaces, water columns, sediments, and affecting marine animals. The obtained results offer a solid basis for upcoming investigations related to this public health concern.
Dry-fermented sausage, salsiccia sarda, a traditional product of Sardinia, is included in the catalog of Italian traditional foods. Some producing plants petitioned for an analysis of the viability of prolonging the shelf-life of vacuum-packaged items by up to 120 days. Three separate batches of Sardinian fermented sausage, resulting in a total of 90 samples, underwent production at two facilities, namely A and B. At the initial time point (T0) and every 30 days thereafter for four months (T30, T60, T120), all samples of the packaged product were subject to analysis for physicochemical properties, total aerobic mesophilic count, Enterobacteriaceae levels, Listeria monocytogenes presence, Salmonella species identification, mesophilic lactic acid bacteria levels, and coagulase-positive Staphylococcus counts. Concentrating on this, sampling was performed on surfaces that came into contact with food and surfaces that did not come into contact with food at both production plants. Each analysis time point was subject to sensory profile analysis. The pH values for the producing plants A and B, at the end of the extended shelf life, were 590011 and 561029, respectively. Water activity levels at T120 were 0.894002 for plant A and 0.875001 for plant B, signifying production differences. Of the 45 samples examined from producing plant A, 733% (33) contained L. monocytogenes, with an average concentration of 112,076 log10 CFU/gram. Plant B's production was completely free of Listeria monocytogenes. A significant detection of Enterobacteriaceae occurred in 91.1% (41/45) of samples from producing plant A, averaging 315,121 log10 CFU/g. Plant B samples showed a presence in 35.5% (16/45), with a mean of 72,086 log10 CFU/g. The investigation yielded no evidence of Salmonella or Staphylococcus aureus. Environmental contamination by L. monocytogenes was highest at the bagging table (contact surface) and processing room floor drains (non-contact surface), both sites registering a prevalence of 50% (8 positive samples each from a set of 16). The sensory analysis at 30 days exhibited peak overall sensory quality; in addition, noticeable variations were seen in visual, tactile, olfactory, gustatory, and textural attributes across the samples over time, with a significant diminution occurring by day 120. Ultimately, the sensory appeal and quality of the vacuum-sealed Sardinian fermented sausage remained unaffected until the 120-day shelf life mark. Nonetheless, the potential for Listeria monocytogenes contamination necessitates a rigorous approach to hygiene throughout the entire technological process. During the control procedure, environmental sampling demonstrated its usefulness as a verification tool.
Usually, the food business operator is responsible for establishing the shelf-life of food products, barring unusual circumstances. Years of discussion amongst actors within the food chain regarding this period's duration have now been elevated to critical importance due to the recent confluence of economic, financial, environmental, and health crises, which have undeniably influenced consumption choices and food waste. While durability isn't mandatory for some food categories—like those not for direct consumption—this discussion brings up potential questions about re-examining the manufacturer's initial standards, especially regarding the need to uphold consumer health and hygiene assurances. Along with the growing demand from consumers for reliable information, European authorities have initiated a public consultation on the correct interpretation and public perception of mandatory labeling requirements, such as 'use by' or 'minimum durability date,' as specified by Article 9 of Regulation (EU) No. 1169/2011. These often-misunderstood terms are crucial to implementing regulations aimed at reducing food waste. The European Union's recent legislative initiatives, coupled with the case law of recent years, have compelled judges to adhere to the food safety guidelines established in Regulation (EC) No. 178, from 2002, thereby improving the focus on the analysis, assessment, and risk management across the whole production network. To bolster the possibility of extending the lifespan of food products, this work offers technical and legal frameworks, ensuring the well-being of consumers.
Through diverse food channels, microplastics (MPs) enter the human body, thus constituting a significant threat to food safety. High microplastic contamination risk for bivalves, stemming from their filter-feeding, translates to a hazard for consumers who eat them raw or cooked whole. Samples of mussels (Mytilus galloprovincialis) and oysters (Crassostrea gigas) sold in Apulia were analyzed, and this research work discovered, measured, identified, and categorized microplastics present within them. Analysis of mussel and oyster samples revealed 789 and 270 plastic particles respectively, spanning a size range of 10 to 7350 micrometers. In both species, the most prevalent findings were fragments between 5 and 500 meters in size. Mussels exhibited blue coloration prominently, whereas oysters displayed transparency. Debris in mussels was largely composed of polyamide and nylon polymers, contrasting with chlorinated polypropylene, which was the predominant debris type in oysters. Mussels and oysters bought at fish markets are shown by these results to contain microplastics. Medical dictionary construction Assessing the impact of marketing stages on microplastic contamination in bivalves requires further research to accurately determine the associated human health risks stemming from consumption. Microplastic contamination sources vary.
Italian samples of European squids (Loligo vulgaris) and flying squids (Todarodes sagittatus) from the northern Adriatic Sea were analyzed to determine the concentration of lead (Pb), cadmium (Cd), and total mercury (Hg). The Italian people's risk of ingesting potentially hazardous metals present in these products was also assessed. Compared to European squids, flying squids accumulated significantly higher levels of total mercury, specifically three times higher. Furthermore, cadmium concentrations were a hundred times greater in flying squids. This resulted in over 6% of Hg samples and 25% of Cd samples surpassing the maximum permissible limits set forth by current legislation.