5a). This relatively small growth must have been due to organic compounds in the culture supernatant of strain AH-1N, which have not been identified

so far. These results indicated that GlcNAc released from chitin by the chitinolytic enzymes of strain AH-1N was most likely the main growth substrate for strain 4D9 in the co-culture. As GlcNAc could not be detected in the supernatant of single cultures of strain AH-1N with embedded chitin, this bacterium apparently exhibited a tight coupling of polymer hydrolysis and GlcNAc uptake. To interfere with this tight coupling, strain 4D9 had to actively integrate into the biofilm for establishing a close contact to zones of chitin hydrolysis and GlcNAc release. This was supported by the fact that in the presence of strain AH-1N, strain 4D9 grew PKC inhibitor mainly in the biofilm fraction (Fig. 2a), while it grew mainly in the suspended fraction when incubated in cell-free supernatant only (Fig. 5a,b), indicating that there was no selective pressure for biofilm formation in the absence of strain AH-1N. As the growth rate with GlcNAc of strain AH-1N (μ = 0.133 h−1) was about three times higher than the growth rate of strain 4D9 (μ = 0.046 h−1) (Fig. 4), strain 4D9 must be more efficient in the uptake of GlcNAc than strain AH-1N to be able to intercept

GlcNAc. This would decrease the rates of growth and of chitinolytic Selleckchem Vemurafenib enzyme production of strain AH-1N and Rolziracetam could explain the observed delay of chitin degradation in the co-culture compared to the single culture of strain AH-1N. Altogether, integration into the biofilm for exploiting chitinolytic enzymes of strain AH-1N could serve as a strategy of strain 4D9 to overcome its inability to degrade embedded chitin itself. Aeromonas hydrophila

strain AH-1N as an enzyme-releasing bacterium has to find a trade-off between the benefit of accessing embedded polymers and the risk of being exploited, while Flavobacterium sp. strain 4D9 as a bacterium with cell-associated enzymes has to find a trade-off between the benefit of avoiding exploitation and the risk of limited access to embedded polymers. In co-culture, the outcome of these contrasting trade-offs was the formation of a mixed-species biofilm on the chitin-containing particle. Despite being exploited, enzyme-releasing bacteria like strain AH-1N occupy a stable ecological niche, in particular in nutrient-limited environments, as the release of extracellular hydrolytic enzymes is an essential prerequisite for making obstructed organic substrates bioavailable. Bacteria with cell-associated enzymes like strain 4D9 or other Bacteroidetes must develop strategies to act as opportunists or cheaters.