0 CHRB2004 Feces, healthy human A + HM_536947.0 CHRB2011 Feces, healthy human A + HM_536948.0 CHRB2050 Feces, diarrheic human A + HM_536949.0 CHRB2167 Feces, diarrheic human B + n/a CHRB2370 Feces, diarrheic human B + HM_536950.0 Ro 61-8048 purchase CHRB2691 Feces, diarrheic human B + HM_536951.0 CHRB2880 Feces, diarrheic human B + n/a CHRB3152 Feces, diarrheic human B W HM_536952.0 CHRB3235 Feces, healthy human X W HM_536954.0 CHRB3287 Feces, healthy human A + HM_536955.0 CHRB3290 Feces, healthy human A + HM_536956.0 https://www.selleckchem.com/products/mm-102.html CHRB3559 Feces, diarrheic human B + n/a CHRB3612 Feces, diarrheic human B + n/a LMG7788 Type strain, gingival sulcus A + DQ_174166.1 a Genomospecies determined using PCR assay for C. concisus

23S rRNA gene. A/B indicates amplification with primer sets for both genomotype A and B. × indicates lack of PCR amplification with either primer set. b + indicates PCR amplification of cpn60 gene; w indicates weak PCR amplification. c Near full-length 16S rRNA gene sequence. AFLP analysis indicated considerable genetic variability existed among the C. concisus isolates (Figure 1). Reproducibility between duplicate independent analyses of each isolate was 93.1 ±

3.6% (mean ± SD; Additional file 1). The isolates clustered into two phylotypes distinguished from each other at the 34% similarity level. All isolates assigned to AFLP cluster 1 belonged to genomospecies A and included the type strain plus Cilengitide clinical trial Org 27569 five isolates that were obtained from healthy (n = 4) and diarrheic (n = 1) humans. Of the seventeen isolates assigned to AFLP cluster 2, 94% (16/17) were isolated from diarrheic stools, and 71% belonged to genomospecies B (n = 12) while 17% belonged to genomospecies A/B (n = 3), 6% belonged to genomospecies A (n = 1), and one isolate was unassigned. Figure 1 Dendrogram of AFLP profiles derived using the unweighted-pair group average linkage of Pearson-product-moment correlation coefficients from 22 Campylobacter concisus fecal isolates (designated CHRB) and the type strain (LMG7788). The bar indicates percentage similarity. LMG, Culture

Collection of the Laboratorium voor Microbiologie, Gent, Belgium. H, healthy humans. D, diarrheic humans. T, type strain. GS, genomospecies as determined by PCR assay of the 23S rRNA gene (2). A, genomospecies A. B, genomospecies B. A/B, indicates positive PCR for both genomospecies A and B. X, indicates negative PCR for both genomospecies A and B. cpn, C. concisus-specific cpn60 PCR. +, positive PCR. W, weak positive PCR. -, negative PCR. Adherence, invasion, and translocation All C. concisus isolates exhibited comparable epithelial adherence to that of C. jejuni 81-176 (Table 2). The mean adherence of isolates belonging to genomospecies A did not differ from that of isolates belonging to genomospecies B (6.00 ± 0.08 log10 CFU/ml, n = 6 versus 6.28 ± 0.20 log10 CFU/ml, n = 5, respectively; P = 0.20).