001) and positively associated with the degree of differentiation of tumor (P < 0.001) in gastric carcinoma. Each of them was have significant difference in statistically. Conclusion: The expression of PIAS3 gene is reduced in gastric cancer, which may revealed an inhibitive effect of PIAS3 on tumor growth. Key Word(s): 1. PIAS3; 2. gastric cancer; 3. non-tumor tissue; 4. immunohistochemistry; Presenting Author: LIN TAO Additional Authors: HAIXING JIANG Corresponding Author: HAIXING JIANG Affiliations: 1st Affiliated

hospital of Guangxi medical university Objective: Objective To observe the effect of Blastocystis hominis on actin cytoskeleton and its possible mechanism. Methods: Hela cells were cultured by DMEM medium in vitro, then Torin 1 purchase observed the biological characteristics; determinated MTT colorimetry OD value of the growth curve; all objecties were divided into three groups: ① blank control group: Hela cells were cultured alone; ② co-culture group: B.hominis and Hela cells were cultured at the same time; ③ co-cultured + inhibitor group:

B.hominis and Hela cells were cultured at the same time, 0.01% ammonium molybdate was added to Hela cells. Cell cultured in each experimental group were fixed under dynamic inverted phase contrast microscope after 24 h to observe living cells morphological changes. Selleckchem LDE225 Rhodamine – phalloidin was used staining actin cytoskeleton of groups of Hela check details cell. Results: 1. Hela cells were cultured in DMEM medium for adherent growth polygonal. Hela cells formed stable after the third generation, and may be formed on the cell island. 2. Hela cell growth

curve present ‘S’ shape, and went through three growth stages: incubation period, the logarithmic growth phase and stagnation. 3. Results of actin cytoskeleton of Hela cells stained by rhodamine – phalloidin after 24 h showed: ① control group, actin cytoskeleton distributed in the perinuclear area, tension wire structure was visible. ② co-culture group, the content of actin cytoskeleton became less, tension wire structure can not be founded. ③ co-cultured + inhibitor group, the content of the actin cytoskeleton was slightly less, and distributed in the perinuclear area, tension wire structure also was visible. Conclusion: DMEM medium can cultivate the Hela cells morphology, function better. Tanswell insert semi-permeable membrane can cultivate B.hominis and Hela cells better at the same time, and simulate the interaction between B.hominis and cell the in vivo environment. When the B.hominis and Hela cells were co-cultured, B.hominis may secrete acid phosphatase in growth and metabolic processes. It plays a significant role in actin cytoskeleton of Hela cells, and makes actin cytoskeleton decreased markedly and its structure abnormal. Key Word(s): 1. Blastocystis hominis; 2. Hela cells; 3. co-culture; 4.