2-7.6. S. enterica subsp. houtenae is salicin-positive and able to grow in KCB medium, two distinguishing characteristics when compared with S. enterica subsp. enterica. The strain is deposited in the Salmonella Genetic Stock Centre (SGSC), University of Calgary, Canada as S. enterica http://www.selleckchem.com/products/Cisplatin.html subsp. houtenae RKS3027 (= SGSC 3086). Table 1 Classification and general features of S. enterica subsp. houtenae RKS3027 according to the MIGS recommendations [12] Genome sequencing information Genome project history This organism was selected for sequencing on the basis of its phylogenetic position and its serious virulence in humans compared to the reptiles. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession “type”:”entrez-nucleotide”,”attrs”:”text”:”ANHR00000000″,”term_id”:”481053928″,”term_text”:”ANHR00000000″ANHR00000000.

The version described in this paper is the first version, “type”:”entrez-nucleotide”,”attrs”:ANHR01000000″ANHR01000000, and the sequence consists of 97 large contigs. Table 2 presents the project information and its association with MIGS version 2.0 compliance [12]. Table 2 Project information Growth conditions and DNA isolation S. enterica subsp. houtenae strain RKS3027 was grown Luria Broth (LB) medium at 37��C. The DNA was extracted from the cell, concentrated and purified using the Qiamp kit (Qiagen), as detailed in the manual for the instrument. Genome sequencing and assembly The genome of S. enterica subsp.

houtenae RKS3027 was sequenced using the Illumina sequencing platform by the paired-end strategy (2��100bp). The details of library construction and sequencing can be found at the Illumina web site [26]. The final coverage reached 100-fold for an estimated genome size of 4.5 Mb. The sequence data from Illumina HiSeq 2000 were assembled with SOAPdenovo v1.05. The final assembly contained 97 large contigs (>3000 bp) in 59 scaffolds generating a genome size of 4.4 Mb. Genome annotation Genes were predicted using RAST (Rapid Annotation using Subsystem Technology) [27] with gene caller GLIMMER3 [28] followed by manual curation. The predicted bacterial protein sequences were compared with the annotated genes from four available Salmonella genomes, i.e., S. enterica subsp. enterica Typhi P-stx-12, S. enterica subsp.

enterica Heidelberg B182, S. enterica subsp. enterica Typhimurium UK-1 and S. enterica subsp. enterica Typhimurium 4/74 and searched against the Clusters of Orthologous Groups (COG) databases using BLASTP. The BLAST results were filtered with the following parameters: identities >90% and compared length >70%. CGViewer was used for visualization of genomic features [29]. Genome GSK-3 properties The genome of S. enterica subsp. houtenae RKS3027 is 4,404,136 bp long (97 contigs) with a 51.68% G + C content (Table 3 and Figure 2).