, 2013). Flow noise is a form of pseudo-noise caused by turbulence around the hydrophone (Strasberg, 1979), and is not actually present in the environment. While noise from shipping was more dominant than flow noise at both sites DZNeP clinical trial (Fig. 5), flow noise exceeded non-anthropogenic noise levels below ∼160 Hz at the Chanonry site (Fig.

4), and so may influence measurements in areas of low shipping density. Since flow noise decreases with increasing frequency (Strasberg, 1979), higher frequency bands would be progressively less susceptible to flow noise contamination than those at 63 and 125 Hz. Comparison of the proposed 1/3-octave frequency bands with those at 250 and 500 Hz (Fig. 9) indicates that the 250 Hz band may be as responsive to noise exposure from large vessels as the 125 Hz band, and may perform better than the 63 Hz band in shallow water. Although peak frequencies of commercial ship source levels are typically <100 Hz (e.g. Arveson and Vendittis, 2000 and McKenna et al., 2012), low-frequency sound may be rapidly attenuated in shallow water depending on the water depth (Jensen et al., 2011), meaning received ship noise levels may have higher peak frequencies than in the open ocean. The 250- and 500-Hz bands are also likely to contain a greater amount of the noise from small vessels (since their spectra can peak at up to several kHz (Kipple and Gabriele,

2003 and Matzner et al., 2010)), which may be the dominant

source of ship noise in some coastal areas. Inclusion of noise levels at frequencies greater than 125 Hz may therefore be particularly find more informative for MSFD noise monitoring in shallow waters. A wider concern for the efficacy of the MSFD with regard to shipping noise is the proposed focus (Van der Graaf et al., 2012 and Dekeling et al., 2013) of ambient noise monitoring on high shipping density areas. While it is important that the most acoustically polluted waters are represented in noise monitoring programs, it is arguably the case that habitats most at threat from Temsirolimus in vitro anthropogenic pressure should be given greater weight. If noise levels in high shipping areas are to determine whether a member state of the European Union attains ‘Good Environmental Status’, there is a risk that more significant changes to the marine acoustic environment in less polluted areas will be overlooked. Funding for equipment and data collection was provided by Moray Offshore Renewables Ltd., and Beatrice Offshore Wind Ltd. We thank Baker Consultants and Moray First Marine for their assistance with device calibration and deployment, respectively. The POLPRED tidal model was kindly provided by NERC National Oceanography Centre. We also thank Rebecca Hewitt for collating and preparing the weather data, Stephanie Moore for advice on sediment transport, and Ian McConnell of shipais.com for AIS data. N.D.M.