Next, distributions or spectra
of relative frequencies across 92 SNP sites from blood of patients in the HBV-HCC, alcohol-HCC, and control groups were compared to provide the topology of polymorphisms (Fig. 1). The diversity of distribution was Sotrastaurin mouse analyzed by paired t-test and SNPs in HBV-HCC patients apparently showed distinct spectrum from control (p = 0.0001). The SNP distribution in the D-Loop region in alcohol-HCC appeared to be less differentiable from HBV-HCC and control. Table 2 Average SNP frequency in the mitochonrial DNA D-Loop Ruxolitinib ic50 for each group. Control (n = 38) HBV-HCC (n = 49) Alcohol-HCC (n = 10) SNPs/patient 6.7 ± 2.0b 8.5 ± 2.2 8.0
± 1.9 P valuea 0.0002 0.0730 aT test. bMean ± standard deviation Figure 1 Distribution (spectrum) of D-Loop SNPs at 92 sites (x-axis) and their relative frequencies in percentage within each group (y-axis). Paired T-test: p = 0.0001 (HBV-HCC vs. control); p = 0.3416 VS-4718 cost (Alcohol-HCC vs. control); p = 0.2817 (HBV-HCC vs. Alcohol-HCC). When individual SNPs were analyzed between HCC and control, a statistically significant increase of SNP frequency was observed for 16298C and 523del alleles in HBV-HCC (p < 0.05) and for 16293G, 523del, and 525del alleles in alcohol-HCC (p < 0.05) patients (Table 3). The trend was next determined with all 3 groups using t test. Additional SNPs (16266T, 16299G, 16303A, 242T, 368G, and 462T) were significantly associated with the tendency toward the increased risk for alcohol-HCC. In contrast, the 152C allele was correlated with reduced risk, especially for alcohol-HCC. The remaining 81 SNPs were not associated
with either type of HCC. Nucleotidea Control HBV-HCC Alcohol-HCC Trend-p valueb 16266 C/T 37/1 (2.6)c 49/0 (0.0) 8/2 (20.0) 0.0038 d P value 0.4368 0.1058 16293 A/G 38/0 (0.0) 48/1 (2.0) 8/2 (20.0) 0.0042 P value >0.9999 0.0399 16298 T/C 35/3 (7.9) 37/12 (24.5) 9/1 (10.0) Liothyronine Sodium 0.0992 P value 0.0495 >0.9999 16299A/G 38/0 (0.0) 49/0 (0.0) 9/1 (10.0) 0.0123 P value >0.9999 0.2083 16303 G/A 38/0 (0.0) 49/0 (0.0) 9/1 (10.0) 0.0123 P value >0.9999 0.2083 152 T/C 30/8 (21.1) 31/18 (36.7) 10/0 (0.0) 0.0340 P value 0.1130 0.1767 242 C/T 38/0 (0.0) 49/0 (0.0) 9/1 (10.0) 0.0123 P value >0.9999 0.2083 368 A/G 38/0 (0.0) 49/0 (0.0) 9/1 (10.0) 0.0123 P value >0.9999 0.2083 462 C/T 38/0 (0.0) 49/0 (0.0) 9/1 (10.0) 0.0123 P value >0.9999 0.2083 523 A/del 32/6 (15.8) 31/18 (36.7) 4/6 (60.0) 0.0122 P value 0.0302 0.0092 525C/del 30/8 (21.1) 31/18 (36.7) 4/6 (60.0) 0.0483 P value 0.1130 0.