Differential induction of certain AvBDs by the wild type SE and

the pipB mutant was also observed at these times. Among the constitutively and highly VX-809 mouse expressed AvBD genes, infection of COEC with ZM100 (wt) or ZM103 (sipA) resulted in a temporary repression of AvBD4, and AvBD9-11 (≤ 1.5-fold), but not AvBD5 and AvBD12 (Figure 3). Infection of COEC with ZM106 (pipB) had reduced or no suppressive effect on the transcription of AvBD9-11, compared to infections with strains ZM100 and ZM103 (Figure 3). With the moderately expressed genes, infection of COEC with ZM100 (wt) or ZM103 (sipA) had minimal effect (< 1.5-fold) on the expression of AvBD1 and AvBD13-14, whereas ZM106 (pipB) temporarily induced the expressions of these genes at 1 hpi (Figure 4). The expression of another moderately expressed gene, namely AvBD3, was initially suppressed by ZM100, but not ZM106, and then induced by all three SE strains at 4 hpi and 24 hpi (Figure 4). With the minimally expressed genes, AvBD2 and AvBD6 were induced by all SE strains examined. However, the expression levels of AvBD2 and AvBD6 in COEC infected with ZM106 were significantly higher than

that in COEC infected with ZM100 or ZM103 (Figure 5). The expression of AvBD7 and AvBD8 in COEC was minimally check details affected by exposures to ZM100 and ZM103. Compared to the wild type strain and the sipA mutant, ZM106 also induced elevated expression of AvBD7 (Figure 5). Figure 3 Transcriptional changes of constitutively and highly expressed

AvBDs in COEC following infections with SE. Data shown (fold change) are geometric means of three independent experiments ± standard deviation. Open bar, ZM100 (wt); solid bar, ZM103 (sipA); JQEZ5 mw hatched bar, ZM106 (pipB). * indicates that the difference between the transcriptional changes induced by the wild type SE and the mutant is significant (p < 0.05). Figure 4 Transcriptional changes of Dichloromethane dehalogenase moderately expressed AvBDs in COEC following infections with SE. Data shown (fold change) are geometric means of three independent experiments ± standard deviation. Open bar, ZM100 (wt); solid bar, ZM103 (sipA); hatched bar, ZM106 (pipB). * indicates that the difference between the transcriptional changes induced by the wild type SE and the mutant is significant (p < 0.05). Figure 5 Transcriptional changes of minimally expressed AvBDs in COEC following infections with SE. Data shown (fold change) are geometric means of three independent experiments ± standard deviation. Open bar, ZM100 (wt); solid bar, ZM103 (sipA); hatched bar, ZM106 (pipB). * indicates that the difference between the amounts of AvBD transcripts in ZM100-infected COEC and ZM106-infected COEC is significant (p < 0.05).