and so on. High-quality of written English, Not ideal for publication except if extensively edited. Authors response, This manuscript is primarily based over the hypothesis that a subset of lncRNAs can be processed into little RNAs therefore also potentially contributing to independent functions. In our analysis, we were in a position to computationally map smallRNA datasets and recommend that a subset of lncRNAs may very well be potentially processed to smallRNAs. The present evaluation with its limitations are not able to exclude this currently being an independent event nor can assign possible biological implications to this observa tion. Our observation hop over to these guys also reveals that there’s a prefer ence in the smallRNAs to map towards the exons of lncRNAs. Extra examination information propose it is a regulated process, with differential expression in tissues.
This helps make us argue that this might propose a coordinated and regu lated method of biogenesis as an alternative to a degradation approach, however at this minute we lack hard proof to prove or disprove this likelihood. We report his observa tion with sufficient datasets to support even further in depth read full report examination and experiments in this course. We’re also aware of epigenetic signals which each mark genes at the same time as regulate gene expression. We have without a doubt carried out extensive evaluation in these lines, but are obviously past the scope of this manuscript. I thank the authors for placing a lot get the job done into addressing my worries. Technical Comments, The authors have now presented a finish dataset of compact RNA clusters overlapping Gencode LncRNAs. This file is Supplementary File 3.
You will find some troubles still, one The file is prone to be used by bioinformaticians, to ensure that a text file tab delimited file might be more prac tical than a pdf. two Even in instances in which various clusters overlap one particular lncRNA, a single place for the clusters is offered, whereas it truly is needed to specify the areas and IDs of All the clusters. 3 The place given for your lncRNA is exon posi tions. It could extra logical to provide thestart and finish place of your RNA transcript. Figure 1 remains a crude screen shot from Ensembl that is just about incomprehensible to me. It is actually wonderful that the authors estimated the overlap rate of little RNA clusters in lncRNA exons and introns. An extra good control will be counting the price of overlap of small RNA clusters within the OPPOSITE strand of lncRNA exons and introns. Total I even now have doubts concerning the worth of this get the job done to thescientific community, since it presently stands that is unfortunate mainly because the authors have identified an extremely timely contemporary question within the area, whether or not and which lncRNAs serve as precursors to modest RNAs.