For a first insight

into this large data set, a screening for interesting mutants was done by a pattern search, focusing on mutants with changes in specific pathways. We show that our transposon selleck inhibitor mutant library is not biased with respect to insertion points. A comparison of the results for specific mutants with previously published metabolic results on a deletion mutant of the same gene confirmed the concept of high-throughput metabolic profiling. Altogether the described method could be applied to whole mutant libraries and thereby help to gain comprehensive information about genes with unknown, hypothetical and known functions.”
“Paroxysmal nocturnal hemoglobinuria (PNH) is a rare acquired stem cell disorder associated with periodic hemolytic events. This benign clonal condition is caused by find more the abnormal X-linked phosphatidylinositol glycan class A (PIGA) gene and has been associated with cytopenias and thrombosis. Recent improvements in PNH diagnostics relate to technical advances in flow cytometry (FCM), which can detect PNH cells at about 0.01% of total cells. Also, limitations of fluorescent inactivated aerolysin (FLAER) for measurement of the RBC clone have been recognized.

Earlier methods involved immunological techniques associated with complement-mediated RBC lysis. These tests, including both Ham’s acid hemolysis test (HT) and the sucrose lysis test (SLT), can detect PNH cells at <5% of total cells. These lytic techniques have been replaced by multi-color FCM with monoclonal antibodies (mAbs), such as CD 55 and CD 59, and FLAER, which both bind to the normal glycophosphatidylinositol (GPI)-anchors, or GPI-anchor

proteins.”
“In the present study, an enzyme-linked immunosorbent assay (ELISA) standardized with vesicular fluid of Taenia solium cysticerci was used to screen for IgG (total and subclasses) and IgE antibodies in cerebrospinal fluid (CSF) samples from patients with neurocysticercosis showing intrathecal production of specific IgG antibodies and patients with other neurological disorders. The following results selleck were obtained: IgG-ELISA: 100% sensitivity (median of the ELISA absorbances (MEA)=1.17) and 100% specificity; IgG(1)-ELISA: 72.7% sensitivity (MEA=0.49) and 100% specificity; IgG(2)-ELISA: 81.8% sensitivity (MEA=0.46) and 100% specificity; IgG(3)-ELISA: 63.6% sensitivity (MEA=0.12) and 100% specificity; IgG(4)-ELISA: 90.9% sensitivity (MEA=0.85) and 100% specificity; IgE-ELISA 93.8% sensitivity (MEA=0.60) and 100% specificity. There were no significant differences between the sensitivities and specificities in the detection of IgG-ELISA and IgE-ELISA, although in CSF samples from patients with neurocysticercosis the MEA of the IgG-ELISA was significantly higher than that of the IgE-ELISA. The sensitivity and MEA values of the IgG(4)-ELISA were higher than the corresponding values for the other IgG subclasses.