The benzene ring containing nitrogen compounds shows promising results and it is consider as important pharmacophore of the lead compounds specifically X-OCH3 increasing the binding affinity more to the active site compared
with X-CH3. The nature of interaction and its conformation with dock score is shown in the Table 2. Hence these compounds can be further analyzed invitro and invivo to check it’s potent against MAP kinases. BTZ-4a = 1H NMR PF-01367338 datasheet (300 MHz, CDCl3) δ: 7.18–8.14 (m, 8H, Ar–H), 3.28 (s, 2H), 2.15 (s, 6H); 13C NMR (300 MHz, CDCl3) δ: 166.92, 151.37, 136.01, 132.88, 130.80, 130.66, 126.81, 126.03, 125.86, 125.74, 123.56, 83.26, 42.31, 15.03; ESI-MS, m/z calcd. for C17H16BrNS3 410.41 found [M]+ 410. BTZ-6a = 1H NMR (400 MHz, CDCl3) δ: 7.20–9.32 (m, 7H, Ar–H), 3.42 (s, 2H, CH2), 2.39 (s, 3H, CH3), 2.16 (s, 6H, 2CH3); 13C NMR (300 MHz, CDCl3) δ: 166.89,
151.50, 149.94, 148.51, 137.36, 135.83, 135.07, 134.45, 125.64, 125.12, 123.05, 122.34, 82.69, 42.03, 20.99, 14.50; ESI-MS, m/z calcd. for C17H18N2S3 346.53 found [M+H]+ 347.5. BTZ-6b = 1H NMR (300 MHz, CDCl3) δ: 7.12–9.21 (m, 7H, Ar–H), 3.91 (s, 3H, OCH3), 3.21 (s, 2H, CH2), 2.18 (s, 6H, 2CH3); 13C NMR (300 MHz, CDCl3) δ: 166.35, 157.25, 151.42, 148.81, 136.23, 130.30, 124.32, 124.16, 112.94, 112.38, 82.99, 56.31, 41.80, Inhibitor Library in vitro 14.40; ESI-MS, m/z calcd. for C17H18N2OS3 362.53 found [M+H]+ 363.5. BTZ-19 = 1H NMR (400 MHz,CDCl3) δ: 7.05–7.91 (m, 7H, Ar–H), 3.83 (s, 3H, OCH3), 3.25 (s, 2H, CH2), 2.42 (s, 3H, CH3), 2.15 (s, 6H, 2CH3); 13C NMR (400 MHz, CDCl3) δ: 167.45, 156.51, 145.89, 141.11, 136.56, 129.20, 127.39, 126.70, 124.14, 119.06, 116.73, 82.23, 55.64, 42.07, 21.45, 14.70; ESI-MS, m/z calcd. for C19H21NOS3 375.57 found [M+H]+ 376.5. BTZ-20 = 1H NMR (400 MHz, CDCl3) δ: 7.14–8.15 (m, 12H, Ar–H),
3.85 (s, 3H, OCH3), 3.30 PD184352 (CI-1040) (s, 2H, CH2), 2.17 (s, 6H, 2CH3); 13C NMR (400 MHz, CDCl3) δ: 167.17, 156.64, 145.82, 143.36, 140.28, 138.09, 128.86, 127.91, 127.79, 127.09, 126.80, 124.22, 119.10, 116.77, 113.20, 101.56, 82.33, 55.66, 42.13, 14.72; ESI-MS, m/z calcd. for C23H21NS3 437.09 found [M+H]+ 438.8. BTZ-14a = 1H NMR (400 MHz, CDCl3) δ: 7.12–7.65 (m, 6H, Ar–H), 3.12 (s, 2H, CH2), 2.35 (s, 3H, CH3), 2.12 (s, 6H, 2CH3); 13C NMR (400 MHz, CDCl3) δ: 161.91, 151.75, 143.37, 136.25, 134.75, 131.34, 130.58, 129.53, 125.83, 123.46, 81.28, 43.79, 21.05, 14.98; ESI-MS, m/z calcd.