In-hospital mortality was 23%. The median duration of VAC therapy was 23 days (range, 4-61 days) and the median number of VAC changes per
patient was 6 (range, 2-14 days). Infection control and successful chest cavity closure was achieved in all surviving patients. One adverse VAC treatment-related event was identified (5%). Conclusions: The intrathoracic VAC application is a safe and efficient treatment of infected postpneumonectomy chest cavities and allows the preservation of chest wall integrity.”
“A series of new fluorescent symmetric dimeric bisbenzimidazoles DBP(n) bearing bisbenzimidazole fragments joined by oligomethylene linkers with a central 1,4-piperazine residue were synthesized. The complex formation of DBP(n) in the DNA minor groove was demonstrated. The DBP(n) at micromolar concentrations inhibit in vitro eukaryotic DNA topoisomerase I and prokaryotic DNA methyltransferase (MTase) A-1210477 research buy M.SssI. The DBP(n) were soluble well in aqueous solutions and could penetrate cell and nuclear membranes and stain DNA in live cells. The DBP(n) displayed a moderate effect on the reactivation of gene expression. (C) 2015 Elsevier Ltd. All rights reserved.”
“In this study, we aim to screen meta
stasis-related proteins in human lung squamous carcinoma (LSC) using laser capture microdissection and a proteomic approach. Twenty two differential proteins were identified from pooled microdissected primary LSC and matched lymph node (LN) metastatic tissues. Expression Cell Cycle inhibitor of the differential protein 14-3-3 sigma was determined by Western blotting and immunohistochemistry. In cell invasion assay, down-regulated 14-3-3 sigma by siRNA increased in vitro invasive ability of HTB-182 and A549 cells, up-regulation of 14-3-3 sigma by pcDNA3.0/14-3-3 sigma decreased in vitro invasive ability of HTB-182 and A549 cells. The data suggest that 14-3-3 sigma is a potential LN metastasis-related protein in LSC, and its dysregulation might play an important
role in the LN metastatic process of LSC. (C) 2009 Elsevier Ireland Ltd. All rights reserved.”
“A solution chemical method utilizing CCI-779 ethylene glycol as solvent has been developed to prepare the ceramics of (1-x)Pb(Mg1/3Nb2/3)O-3-xPbTiO(3)[(1- x)PMN-xPT] from a precursor powder that can be pressed and fired in one step to produce high quality ceramics with excellent piezoelectric properties. The ceramics reach a relative density of up to 97% of the theoretical value after direct calcinations. This high density is achieved without the need of additional sintering after calcination which is usually required in conventional solid state syntheses to produce ceramics. The ceramics exhibit a unipolar piezoelectric coefficient d(33) of 848 pC/N, which is one of the highest values for any unmodified/untextured binary systems reported to date. Since the piezoelectric properties depend on composition and electric field, the effect of poling conditions was investigated.