In this study we found that the pSS salivary profile was characterised by a decrease in many secretory proteins, an increase of proteins related to the autoimmune response and an increase of proteins related to systemic and local inflammation. On the other hand, as expected, subjects with RA sSS or SSc sSS shared a selleckchem number of salivary biomar kers, whereas subjects with sicca complaints but without SS were characterised Inhibitors,Modulators,Libraries by a proteomic profile much closer to that of healthy subjects with the exception of an increase of proteins related to inflammation and a decrease of secretory proteins, such as PIP and SPLUNC 2. In fact, the latter Inhibitors,Modulators,Libraries was still significantly increased in patients with non SS sicca with respect to pSS patients while PIP showed a one fold variation when compared to pSS.

The similarities and the differences between the groups were clearly displayed in the dendo gram representation. The dendogram showed, in particu lar, that pSS and RA sSS were Inhibitors,Modulators,Libraries so close in value that together they made a cluster easily distinguishable from the other groups. Subjects with Inhibitors,Modulators,Libraries SSc sSS created a second, different cluster which was in between the others, showing not only a reduction of many acinary proteins and an increase of proteins related to lymphocyte activation, but also an increased expression of G3PDH which was closer to the profiles of non SS sicca syndrome patients and healthy volunteers. In turn, healthy volunteers and patients with non SS sicca syndrome were apparently very similar and represented a third cluster. These results confirmed our previous preliminary data and pre existing literature.

However, in this study, the expression of salivary biomarkers Inhibitors,Modulators,Libraries was also verified in an independent cohort of healthy volunteers and pathological controls, distinct from the derivation cohort, by using additional antibody detection tests. This allowed Tenatoprazole? us to conclude that a panel of candidate biomarkers rather than a single specific pro tein may apparently be able to better distinguish pSS from healthy volunteers and other pathological disorders. In the present study, we first described 15 proteins which could represent candidate biomarkers to be included in a potential diagnostic panel for pSS. Some of them appeared to be significantly increased or decreased only in comparison with healthy volunteers and, therefore, their diagnostic role for pSS remains controversial.