The SRA–DNA interaction may serve as an MCC950 research buy anchor to keep UHRF1

at a hemi-methylated CpG site where it recruits the DNMT1 for DNA methylation maintenance [9, 11]. Thus, UHRF1 plays a fundamental role in the inheritance EPZ5676 cost of the DNA epigenetic marks from the mother cell to the daughter cells. It also appears that preventing the transmission of these marks via knock-down of UHRF1 leads to an activation of pro-apoptotic pathways [9, 12–16]. In agreement with this hypothesis, UHRF1 down-regulation has been shown to inhibit cell growth and induces apoptosis of colorectal cancer through p16INK4A up-regulation [17]. Some bioactive plants components have been shown to have cancer inhibition activities by reducing DNA hypermethylation of key cancer-causing genes

through their DNA methyltransferase (DNMT) inhibition properties [18]. In this context, recently we found that the epigallocatechin-3-gallate (EGCG), a natural anti-cancer drug induces G1 cell arrest and apoptosis in Jurkat cells by down-regulating UHRF1 and DNMT1 expression, with subsequent up-regulation of p16 INK4A gene [19]. L. guyonianum has been used in traditional medicines to treat gastric infections. It has also been employed as an anti-bacterial drug in the treatment of bronchitis [20]. L. feei has been similarly used in the treatment of bronchitis and stomach infections [21]. Previous investigations revealed that methanol extract from L. feei leaves contained potential anti-fungal Selleck Rabusertib constituents that could be PIK3C2G employed against Candida albicans and anti-bacterial constituents useful against E. coli[22]. More recently, our laboratory demonstrated that L. guyonianum aqueous gall extract was able to induce splenocyte proliferation and to stimulate macrophage activation [23]. Chemical investigation of Limoniastrum genus has been reported in literature. Indeed, bioguided fractionation of leaves

extract from Limoniastrum feei led to the isolation of several polyphenolic constituents such as Gallic acid, Epigallocatechin gallate, Quercetin and Myricetin [24]. A subsequent article noted that ethyl acetate extract of L. guyonianum contained gallocatechin, epigallocatechin, and epigallocatechin-3-O-gallate [25]. Several groups have reported that epigallocatechin gallate exhibited antitumor effects that were discovered from various cancer cell lines, animal models and clinical studies [26]. For example, in vivo studies showed that epigallocatechin gallate administration decreased H1299 xenograft tumor growth [27]. Furthermore, myricetin treatment significantly inhibited the tumor growth on T24 bladder cancer xenografts model [28]. In the same way, it was demonstrated that gallic acid plays a critical role as an anticancer agent in vivo by decreasing MNNG/HOS xenograft tumor growth in Balb/C mice [29].