, 2011). TLR4 receptors may indirectly contribute to the endothelial barrier dysfunction by activation of independent signaling pathways that release proinflammatory cytokines. TLR2 and TLR4 are receptors for HMGB1, a nuclear transcription factor released with a lag phase of 8–32 h after endotoxin challenge by necrotic and inflammatory cells, and associated with endothelial cell cytoskeletal rearrangement

and barrier disruption (Wang et al., 1999; Wolfson Gefitinib concentration et al., 2011). However, we observed that inoculation of B. jararacussu venom enlarged the regional lymph node and increased cellularity which was evident in both groups (TLR-deficient and wild-type mice) since 3 DPI, but such effect only persisted in the Tacrolimus manufacturer TLR4-deficient at later stages (21 DPI). The common sequelae from bothropic poisoning is loss of muscle mass due to direct action of myotoxic phospholipases on muscle plasma membrane inducing massive muscle necrosis (Barbosa

et al., 2009; Doin-Silva et al., 2009). Failure to effective muscular regeneration may be related to death of satellite cells (Queiroz et al., 1984), although experimental evidence showed that regeneration was also verified after treatment with myotoxins indicating that in some circumstances satellite cells are still resistant to venom toxins (Harris et al., 2003). In the present study it utilized the crude venom from B. jararacussu, which contains a highly complex mixture of proteases Clostridium perfringens alpha toxin including phospholipases, metalloproteases and general cytotoxins capable to initiate cycles of degeneration and regeneration in skeletal muscles ( Escalante et al., 2011). Thus the loss of muscle mass induced by the venom of B. jararacussu can be mainly attributed to thrombosis and ischemia, which may prevent the activation of satellite cells through

cytokines released by inflammatory cells, or decrease the access of hematopoietic stem cells to the injury site ( Charge and Rudnicki, 2004). In our model, both strains showed loss of muscle mass in the final stages of regeneration. Skeletal muscle remodeling after injury is accompanied by a constant turnover of extracellular matrix components, important in the maintenance of myofiber functional integrity. MMP9 is a protease produced mainly by inflammatory and activated satellite cells (Kherif et al., 1999). A previous study showed increased MMP9 activity 6 h after intramuscular inoculation of bothropic venom in the gastrocnemius muscle (Rucavado et al., 2002) although C3H/HeJ mice subjected to myocardial injury had reduction of MMP9 activity (Caso et al., 2007). In the present study it was also observed 3 days after intramuscular injection of B.