3 Hz for different BLP frequencies (δ, θ, α, β, γ) and different RSN. Repeated-measures ANOVAs with network (visual, auditory, dorsal attention) and condition (fixation and movie) as main VX-770 clinical trial factors showed a significant reduction of the total interdependence in movie as compared

to fixation. This reduction was significant in α BLP (main effect condition (F1,33 = 14.19, p < 0.001, pη2 = 0.30)), in β BLP (main effect condition (F1,33 = 5.21, p = 0.04, pη2 = 0.12)) (Figure 2B) while it did not reach significance in δ (F1,33 = 3.45, p = 0.07), θ (F1,33 = 1.24, p = 0.27) and γ BLP (F1,33 = 1.24, p = 0.30) (Figure S2). The decrement in total interdependence was consistent across different RSN (interaction network × Condition, both for α and β band [all p values > 0.05]). Next, we considered modulation of total interdependence estimated across-RSN nodes, as opposed to within-RSN as in the previous analysis. Again, there was a significant reduction during movie with respect to fixation in α BLP (main effect condition (F1,33 = 5.66, p = 0.02, pη2 = 0.15)). Importantly, this effect was consistent across networks (interaction network × condition, p > 0.05). No significant modulation was detected in the other frequency bands (all p values > 0.05) (Figure S3). Overall, in agreement

with previous MEG reports (Brookes et al., 2011b, de Pasquale et al., 2010, de Pasquale et al., 2012 and Hipp et al., 2012), functional coupling between nodes of RSN was characterized by slow fluctuations of BLP at about 0.1 Hz. Watching a movie leads to Ketanserin an overall decrement of inter-nodal interaction at frequencies < 0.3 Hz, mainly in www.selleckchem.com/products/fg-4592.html the α BLP, both within and across networks. Hence, visual stimulation seems to promote a reduction of functional connectivity as captured by α (and β for within-RSN) BLP interactions. Next, we considered whole-brain changes in the topography and strength of BLP correlation induced by movie watching. To map voxelwise modulation, BLP correlation maps were computed between a RSN node and the rest of the brain assuming the stationarity

of BLP correlation (de Pasquale et al., 2010), using the Pearson product moment formula (Experimental Procedures). Individual node Z score correlation maps were averaged across runs and subjects to compute group-level maps in each condition (fixation, movie). To minimize the effect of field spread, difference maps between conditions were computed and then averaged across RSN nodes to yield voxel-wise BLP difference correlation maps between movie and fixation (Supplemental Information for details). Figure 3A shows α BLP correlation changes from fixation to movie obtained by averaging all nodes in the visual network. Note the widespread decrement of correlation broadly across occipital visual cortex, bilaterally, extending into posterior parietal cortex (dorsal attention network) and temporal cortex (auditory network), especially in the right hemisphere.