On days 7, 14, 21, 35, and 52 after transplant, 5 mice per group were killed and evaluated by histologic assessment and scanning electronic microscopy for reepithelialization and fibrosis of tracheal grafts.
Results: Mean diameter of gelatin microspheres was 107 mu m. Microspheres could not be fully degraded until 35 days after transplant in vivo. On days 7, 14, and 21, epithelium score and ratio of lamina propria to tracheal cartilage were not statistically different between mice with epidermal growth
factor-loaded gelatin microspheres and other groups. On days 35 and 52, selleck chemical however, epithelium score was higher and ratio of lamina propria to tracheal cartilage was lower in epidermal growth factor-loaded gelatin microsphere recipients; these mice also had almost complete differentiation of regenerated epithelium into BTK inhibitor ciliated columnar epithelium on days 35 and 52, earlier than in other groups.
Conclusions: Gelatin microspheres act as a functional vector for
epidermal growth factor. Sustained local application of epidermal growth factor could accelerate reepithelialization of tracheal allografts. (J Thorac Cardiovasc Surg 2010; 140: 209-15)”
“The present paper reviews the enzymes catalyzing conversion of N alpha-benzyloxycarbony l-L lysine (N alpha-2-Llysine) to N alpha-benzyloxycarbonyl-L-aminoadipic acid (N alpha-Z-I-AAA) in fungal Parvulin and bacterial strains A spergillus niger AKU 3302 and Rhodococcus sp AIU Z-35-1 converted N alpha-Z-L lysine to N alpha-Z-L-AAA via Notbenzyloxycarbonyl-L-aminoadipate-delta-semialdehyde (N alpha-Z-L-AASA) However, different enzyme combinations were involved in the N alpha-Z-L-lysine metabolism of both strains A niger strain converted N alpha-Z-L-lysine to N alpha Z-L-AASA by amine oxidase, and the resulting N alpha-Z-L-AASA was converted to N alpha-Z-LAAA by an aldehyde oxidase In the Rhodococcus
strain, conversion of N alpha-Z-L-lysme to N alpha-Z-L-AASA was catalyzed by L-specific amino acid oxidase The resulting Na-Z-L-AASA was converted to N alpha-Z-L-AAA by an aldehyde dehydrogenase The present paper also describes characteristics of new enzymes obtained from those strains”
“Objective: In cardiac cell therapy almost every cell type tested experimentally has yielded some benefit. However, there is a lack of studies directly comparing the function of various stem/progenitor cell populations. This study describes the expansion of peripheral blood CD133(+) cells and compares their functional properties with those of other commonly used human progenitor cell populations.