Recurrent disease often shows tumor progression toward the endometrium or vagina, leading to difficulties in its detection at periodic pelvic examinations.”
“Purpose: To
develop and validate a new, simple, sensitive and accurate high performance liquid chromatographic (HPLC) method for the simulataneous determination of matrine and secnidazole in suppositories.
Methods: The method involved using a SinoChoom ODS-BP C-18 column (5 mu m, 4.6 mm x 200 mm) and mobile phase consisting of acetonitrile-triethylamine (0.05 %) in 0.025 mol/L KH2PO4 (20: 80, v/v). The flow rate was 1 mL/min and detection HDAC inhibitor was monitored at 210 and 311 nm for matrine and secnidazole, respectively. Total run time was 10 min and the column was maintained at 35 degrees C.
Results: The excipients in the suppository did not interfere with the drug peaks. Matrine was eluted at a retention time (RT) of 4.30 min while linearity for the quantification of drug was obtained in the concentration range of 10.0 – 100.0 mu g/mL (r(2) = 0.9991). Secnidazole was eluted at a retention time (tr) of 6.69 min
and linearity for the quantification of the drug was obtained in the concentration range of 10.0 – 150.0 mu g/mL (r(2) = 0.9993). Intra-and inter-day variations were < 1.0 % for both matrine and secnidazole.
Conclusion: The developed HPLC method was validated according to International Conference on Harmonisation (ICH) guidelines and proved to be suitable for the simultaneous CX-6258 determination of matrine and secnidazole in suppositories.”
“Background: The role of diagnostic catheter cerebral angiography has been recently thought MLN8237 solubility dmso to have diminished with the increasing use of noninvasive modalities such as computed tomography angiography and magnetic resonance angiography. Because it is invasive and costly, we sought to elucidate the clinical utility of catheter angiography
by evaluating the indications, incidence of new and clinically meaningful findings, and its utility compared with noninvasive studies. Methods: We performed an observational cross-sectional study of consecutive patients undergoing diagnostic cerebral angiography at an urban tertiary care center. We prospectively recorded procedural indications, the findings of previously performed noninvasive studies, the actual angiogram findings, and whether these were new. We defined a new finding as any novel and clinically meaningful piece of anatomic brain vascular information. Results: Of the 200 consecutive studies over 8 months, 55% were for purely diagnostic purposes, whereas the remaining were for surveillance. The most common indications were subarachnoid hemorrhage, ischemic stroke, intracerebral hemorrhage, cerebral aneurysm, and arteriovenous malformation. New findings were detected in 43% of purely diagnostic angiograms and 32% of surveillance studies. We found false-negative rates of 9.