The majority of dorsal FB neurons in WT flies belonged to the electrically excitable category (57/80 cells or 71%), whereas the majority of dorsal FB neurons in cv-c mutants were electrically silent (25/36 cells or 69%). These figures suggest that Cv-c has a role in setting the intrinsic electrical properties of sleep-promoting neurons. Consistent with this idea, dorsal FB neurons in WT and cv-c mutant flies differed with respect to two parameters that influence the transformation of synaptic or pacemaker currents into membrane

potential changes ( Figure 6A). The input resistance, Rm, determines the size of the voltage change caused by the injection of a fixed amount of current (generated, for example, by the DAPT solubility dmso activation of synaptic conductances); the membrane time constant, τm, defines the temporal window during which multiple inputs can summate. Both Rm and τm were reduced in cv-cC524/cv-cMB03717 mutants in comparison to WT controls ( Figure 6A). Thus, the mutation is predicted to decrease the sensitivity of dorsal FB neurons to synaptic

inputs and curtail opportunities for input integration over time. To investigate the extent to which the membrane properties of members of the dorsal FB neuronal population were modulated in concert, we obtained simultaneous recordings from pairs of neurons http://www.selleckchem.com/products/Lapatinib-Ditosylate.html in the same fly (Figures 6B and 6C). Although spiking and nonspiking neuron types were represented in equal numbers in this data set, the two neurons recorded as part of a pair usually Non-specific serine/threonine protein kinase belonged to the same type (36/44 pairs or 81% concordant; χ2 = 17.82, 1 degree of freedom, p < 0.0001). This, and significant correlations of input resistance

(Figure 6B) and membrane time constant (Figure 6C) between members of a pair, hints that the biophysical properties of different dorsal FB neurons in an individual are coordinated by a common physiological variable, such as the sleep drive of the animal. Recordings from olfactory projection neurons (PNs) in the antennal lobe suggested that coordinated changes in neuronal membrane properties are neither a common feature of all neurons nor a common side effect of the chronic insomnia of cv-c mutants: Rm and τm had statistically indistinguishable average values in cv-c mutants and WT controls ( Figure 6D) and were uncorrelated in pairs of simultaneously recorded PNs ( Figures 6E and 6F). A potential regulatory mechanism thus emerges in which sleep pressure increases the electrical excitability of sleep-promoting neurons in a process that requires the cell-autonomous action of Cv-c.