This http://www.selleckchem.com/products/BI6727-Volasertib.html study was part of a prospective single-centre study on early enteral nutrition vs total parenteral nutrition in AP, where parts of the data on IL-6 and CRP have been published[29]. Venous blood was taken for measurement of plasma levels of TF, FVII, fibrinogen, IL-6 and CRP. Not all markers were measured at all time points in the study. TF and IL-6 were measured at inclusion, after 12 h, and after 1 and 3 d. CRP was measured at inclusion, and after 1 and 3 d. Fibrinogen and FVII were only measured at inclusion in the study. Descriptive data were recorded including age, gender, aetiology, time from onset of pain to inclusion in the study, Acute Physiology and Chronic Health Evaluation (APACHE) II score on day 1 and 3, organ failure, and mortality.

The severity of pancreatitis was assessed according to the Atlanta classification[30]. Blood samples and assays Peripheral blood samples were taken from each patient on study inclusion, at 12 h, and after 1 and 3 d. Admission plasma levels of FVII were analysed, and to detect the prevalence of fibrinolysis and fibrinogen consumption at admission, plasma fibrinogen was analysed. Fibrinogen is an acute phase protein, affected by pathologic proteolysis such as in disseminated intravascular coagulation, where low levels of fibrinogen are to be expected. TF, IL-6 and CRP were analysed at repeated time points during three days after inclusion in the study. Tissue factor and fibrinogen were collected using citrate tubes, and ethylenediaminetetraacetic acid tubes were used for IL-6 and CRP.

All samples were centrifuged at 2200 g for 10 min (3200 r/min, rotor diameter 19.1 cm). The plasma was decanted and stored at -70��C until further analysis. TF and FVII were assessed by enzyme-linked immunosorbent assay (ELISA)-kits according to the manufacturer��s instructions (Assaypro St. Charles, MO, USA). The TF-ELISA recognizes TF-apo, TF and TF-VII complexes. The FVII-ELISA detects free FVII and FVIIa, as well as complexes with TF, TF/factor VII and TF/FVIIa. Fibrinogen was analysed by Sysmex CA-7000 (Sysmex Corporation, Kobe, Japan) according to the operator��s manual. The procedure involves mixing citrate plasma with buffer. After incubation, coagulation was initiated by adding an excess of thrombin. The time between addition of thrombin and coagulation was registered photo-optically and is inversely proportional to the concentration of fibrinogen. IL-6 was measured by an ELISA-kit according to the manufacturer��s instructions (Quantikine, R6D systems Europe, Abingdon, UK). CRP was measured by Cobas 6000 (Roche Anacetrapib Corporation, Basel, Switzerland) according to the operator��s manual.