LB42708? In bone marrow, the mean expression level of ID1 mRNA in stage IV (957��169) was significantly higher than other stages (P=0.003). Specifically, the levels of stages I, II and III were 54��185, 472��208, and 767��205, respectively. To confirm the specificity of ID1, we performed RT�CPCR analysis of six representative cases in each stage, which was very close to the average value (Figure 1C). In addition, sequencing of these transcripts confirmed that it was the product of ID1 (Supplementary Figure 1). Figure 1 The mean value of ID1 mRNA expression normalised GAPDH in bone marrow (A) and peripheral blood (B) according to staging classification. Group stage I consisted of patients with tumours that invaded less than the sub-mucosal layer and no lymph node metastasis …
Expression of ID1 mRNA in peripheral blood of gastric cancer In the peripheral blood samples, there was a significant relationship between the expression level of ID1 mRNA and the progression of gastric cancer cases (Figure 1B). The mean expression level of ID1 mRNA in stage IV (105��15) was significantly higher (P=0.0001) than stages I, II and III (12.4��15.4, 29.6��15.5, and 38.3��16.0, respectively). In addition, there was a significant correlation between the expression of ID1 mRNA in bone marrow and peripheral blood (r=0.23, P=0.002, data not shown). ID1 expression and clinicopathological features of gastric cancer patients We examined the clinicopathlogical significance of ID1 mRNA in samples from bone marrow and peripheral blood (Table 1).
In both bone marrow and peripheral blood, there are significant associations with many cliniopathlogical features such as tumour size and depth of tumour invasion. Especially, in patients with evidence of lymphatic invasion, lymph node metastasis or peritoneal dissemination, we found significantly higher expression of ID1 mRNA in bone marrow samples compared to patients without metastasis. (P=0.001, P=0.001 and P=0.002, respectively, Figures 2A�CC). Similarly, in peripheral blood samples, the cases with lymphatic invasion, lymph node metastasis or peritoneal dissemination had significantly higher expression of ID1 mRNA compared to patients without metastasis. (P=0.02, P=0.02 and P<0.0001 respectively, Figures 3A�CC). Figure 2 Comparison of the ID1 mRNA expression in bone marrow of patients with or without lymphatic invasion (ly) (+: n=156, ?: n= 104; A), lymph node metastasis (N) (+: n=188, ?: n= 79; B) and peritoneal cytology (cy) or peritoneal ... Figure 3 Comparison of the ID1 mRNA expression in peripheral blood of patients with or without lymphatic invasion (ly) (+: n=110, ?: n=68; A), lymph node metastasis (N) (+: n=132, -: n= 50; B) and peritoneal cytology (cy) or peritoneal Drug_discovery …