We did not detect any difference in Septin 9 positivity between left- (96%) and right-sided CRC (94%), so the sensitivity for detecting selleck inhibitor cancer was independent of the location of the tumor. In addition, Septin 9 was by far the most sensitive marker for detecting right-sided tumors. In conclusion, while CRC screening can potentially reduce mortality from colorectal cancer, the current CRC screening tests have unsatisfactory sensitivity and specificity or are highly invasive. Since left-sided CRC is more commonly detected than right-sided CRC by both colonoscopy and blood detection in stool, these screening methods are associated with reduced mortality from CRC arising in the left side of the colon but not from the right side , .
Hence, both improving the compliance of patients and developing more sensitive methods for right-sided CRC are needed. Peripheral blood based methods may raise patient compliance. Our report assesses the possible differences of blood-based SEPT9, gFOBT, and CEA between left- and right-sided CRCs. SEPT9 is a sensitive biomarker for the detection of CRC with the sensitivity of 100% for stage II�CIV CRC. This marker was more sensitive and specific than gFOBT and CEA and did not show any differences between left- and right-sided colon cancers. Hence, the Septin 9 marker may be a safe and useful test for CRC screening. Supporting Information Table S1 Individual results of gFOBT, CEA and SEPT9 for all patinets; NED=no evidence of disease; CRC=colorectl cancer; FOBT=fecal occult blood test; CEA=carcinoembryonic antigen; SEPT9=Septin 9.
(DOC) Click here for additional data file.(380K, doc) Acknowledgments We thank the Endoscopy Unit of Semmelweis University, the Department of Surgery and the Institute for Pathology of the Friedrich-Alexander University Erlangen for their technical assistance. We also thank Anita Nagy for peripheral blood collection and plasma preparation. Furthermore, we thank L��szl�� Hersz��nyi, M��rk Juh��sz, L��szl�� K��nya, Emese Mih��ly, P��l Miheller, Katalin M��llner, Anna M��ria N��meth, Antal P��ter, Hajnal Sz��kely, Rich��rd Szmola (all from the Endoscopy Unit of Semmelweis University) for their work with colonoscopy and biopsy collection. We thank Ibolya Kocsis, the Central Laboratory, and the 1st Department of Pathology and Experimental Cancer Research of Semmelweis University for their work.
Funding Statement This study was supported by Epigenomics AG (Berlin, Germany). The sponsor has partially supplied the reagents for the study, performed technical training and support. Data collection, decision to publish Drug_discovery and preparation of the manuscript were performed by the authors.
A considerable number of lines of evidence have shown that some sets of microRNA (miRNA) act as oncogenes or as tumor suppressors, and that their biogenesis is closely linked to cancer , , . miR-143 and miR-145 have emerged as tumor suppressing miRNAs, particularly for colon cancers.