caeruleus, which forms blue colonies , is the closest known relative of P. daeponensis (Table 7). For this reason, the formation of blue colonies by P. daeponensis DSM 23529T on YTSS medium  observed Brefeldin A ARFs in this study, confirmed by the presence of genes for indigoidine biosynthesis in the genome, is probably of taxonomic relevance. This warrants an update of the taxonomic description of P. daeponensis. Emended description of the species Phaeobacter daeponensis Yoon et al. 2007 The description of the species Phaeobacter daeponensis is the one given by Yoon et al. 2007 , with the following modification. Forms blue colonies when cultivated on YTSS medium. Acknowledgements The authors would like to gratefully acknowledge the assistance of Iljana Schr?der for growing P.
daeponensis cultures and Evelyne-Marie Brambilla for DNA extraction and quality control (both at DSMZ). The work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under contract No. DE-AC02-05CH11231; the work conducted by members of the Roseobacter consortium was supported by the German Research Foundation (DFG) Transregio-SFB 51. We also thank the European Commission which supported phenotyping via the Microme project 222886 within the Framework 7 program.
A representative genomic 16S rRNA gene sequence of strain Z-9701T was compared using NCBI BLAST [4,5] under default settings (e.g.
, considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database  and the relative frequencies of taxa and keywords (reduced to their stem ) were determined, weighted by BLAST scores. The most frequently occurring genera were Thermanaerovibrio (83.8%), Aminomonas (8.5%) and Thermovirga (7.7%) (9 hits in total). Regarding the two hits to sequences from members of the species, the average identity within HSPs was 96.7%, whereas the average coverage by HSPs was 100.5%. Regarding the four hits to sequences from other members of the genus, the average identity within HSPs was 94.9%, whereas the average coverage by HSPs was 96.4%. Among all other species, the one yielding the highest score was T. acidaminovorans (“type”:”entrez-nucleotide”,”attrs”:”text”:”CP001818″,”term_id”:”269099254″,”term_text”:”CP001818″CP001818), which corresponded to an identity of 95.
3% and an HSP coverage of 99.7%. (Note that the Greengenes database uses the INSDC (= EMBL/NCBI/DDBJ) annotation, which is not an authoritative source for nomenclature or classification.) The highest-scoring environmental sequence was “type”:”entrez-nucleotide”,”attrs”:”text”:”AF280820″,”term_id”:”10281596″,”term_text”:”AF280820″AF280820 AV-951 (‘bioreactor clone tbr1-2′), which showed an identity of 94.7% and an HSP coverage of 99.7%.